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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was found to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital purpose in selling cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants were noted but thorough nucleotide sequence details is just not yet readily available.
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Blocking peptides are peptides that bind particularly into the concentrate on antibody and block antibody binding. These peptide commonly consists of the epitope recognized because of the antibody.
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to make sure the very best effects are...
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This guideline outlines more possible optimization of cellular and immunoassay parameters to ensure the absolute best results are...
Antibodies certain to the blocking peptide now not bind for the epitope over the target protein. By comparing the staining through the blocked antibody vs . the antibody alone, one can see which staining is distinct.
Goal Information and facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose users are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation styles which lead for the temporal coordination of each mitotic occasion. Cyclin E varieties a posh with and features as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is also degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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